Ghanem E
Department of Anatomy, University of Gent, Belgium.
Cell Biol Int. 1996 Oct;20(10):681-5. doi: 10.1006/cbir.1996.0090.
Embryonic chick notochords were studied during their metabolically active and involuting periods for the expression of collagen type I and II. The staining was carried out on notochords in vivo at stage 20 and stage 35 and on mesenchyme-contaminated and mesenchyme-free notochords at stage 20, which were cultured in vitro for 6 days. The results show that type II collagen is demonstrable in the notochords, at all the examined stages, both in vivo and in vitro. However, the expression of type I collagen was stage-dependent in vivo and in vitro. At stage 20, the perinotochordal sheath is positively immunostained for collagen type I, but the notochord itself is negative. At stage 35, the perinotochordal sheath as well as the notochord are positively immunostained for collagen type I. The mesenchyme-contaminated and the mesenchyme-free notochords and their sheaths are also positively immunostained for the type I collagen after 6 days in vitro. The current results, at late developmental stages, indicate that the involuting notochords express collagen type I, which seems not to be altered by changing the micro-environment in vivo.
在胚胎期鸡脊索处于代谢活跃和内卷阶段时，研究了I型和II型胶原蛋白的表达情况。在第20阶段和第35阶段对体内的脊索进行染色，并在第20阶段对受间充质污染和无间充质的脊索进行染色，这些脊索在体外培养6天。结果表明，在所有检测阶段，无论是体内还是体外，II型胶原蛋白在脊索中均可检测到。然而，I型胶原蛋白的表达在体内和体外均呈阶段依赖性。在第20阶段，脊索周围鞘对I型胶原蛋白呈阳性免疫染色，但脊索本身呈阴性。在第35阶段，脊索周围鞘以及脊索对I型胶原蛋白呈阳性免疫染色。受间充质污染和无间充质的脊索及其鞘在体外培养6天后对I型胶原蛋白也呈阳性免疫染色。目前在发育后期的结果表明，内卷的脊索表达I型胶原蛋白，这似乎不会因体内微环境的改变而改变。
