Fsihi H, De Rossi E, Salazar L, Cantoni R, Labò M, Riccardi G, Takiff H E, Eiglmeier K, Bergh S, Cole S T
Unité de Génétique Moléculaire Bactérienne, Institut Pasteur, Paris, France.
Microbiology (Reading). 1996 Nov;142 ( Pt 11):3147-61. doi: 10.1099/13500872-142-11-3147.
A continuous 75627 bp segment of the Mycobacterium leprae chromosome spanning the oriC region was sequenced. The gene order at this locus was similar to that found in the replication origin region of many other prokaryotes, particularly Mycobacterium tuberculosis and Streptomyces coelicolor. As in the case of several Gram-positive bacteria, essential genes involved in basic cellular functions, such as DNA or RNA metabolism (dnaA, dnaB, dnaN, gyrB, gyrA, pcnB, recF, rnpA, ssb), cell wall synthesis (ponA, pbpA) and probably cell division (gidB, rodA) were found. Strikingly, the gidA gene was absent from this part of the genome and there was no rRNA operon near oriC. The gyrA gene harbours an intein coding sequence indicating that protein splicing is required to produce the mature A subunit of DNA gyrase. Among the many other noteworthy features were ORFs encoding putative serine/threonine protein kinases and a protein phosphatase, three tRNA genes, one M. leprae-specific repetitive element and a glnQ pseudogene.
对麻风分枝杆菌染色体上跨越oriC区域的一段连续75627 bp片段进行了测序。该位点的基因顺序与许多其他原核生物复制起始区域中的基因顺序相似，尤其是结核分枝杆菌和天蓝色链霉菌。与几种革兰氏阳性细菌的情况一样，发现了参与基本细胞功能的必需基因，如DNA或RNA代谢（dnaA、dnaB、dnaN、gyrB、gyrA、pcnB、recF、rnpA、ssb）、细胞壁合成（ponA、pbpA）以及可能的细胞分裂（gidB、rodA）。引人注目的是，基因组的这一部分没有gidA基因，oriC附近也没有rRNA操纵子。gyrA基因含有一个内含肽编码序列，表明需要蛋白质剪接才能产生DNA促旋酶的成熟A亚基。许多其他值得注意的特征包括编码假定的丝氨酸/苏氨酸蛋白激酶和一种蛋白磷酸酶的开放阅读框、三个tRNA基因、一个麻风分枝杆菌特异性重复元件和一个glnQ假基因。
