Gal-Mor O, Borovok I, Av-Gay Y, Cohen G, Aharonowitz Y
Department of Molecular Microbiology, Biotechnology, The George S. Wise Faculty of Life Sciences, Tel-Aviv University, Ramat Aviv 69978, Israel.
Gene. 1998 Sep 14;217(1-2):83-90. doi: 10.1016/s0378-1119(98)00357-6.
The gene organization was determined in the trxA/B-rnpA region of the Streptomyces coelocolor chromosome, near to the origin of replication, oriC. Previously, we showed that the trxA and trxB genes, coding for thioredoxin and thioredoxin reductase, respectively, occur in S. coelicolor as a gene cluster and are contained on a cosmid H24 that carries oriC and several genes involved in DNA replication. Here we show that the trxA/B locus is positioned approx. 9.4kb from oriC, present the nucleotide sequence of the trxA/B-rnpA region and use sequence analysis to identify the nature of the intervening genes. Seven open reading frames were found, all oriented in the same direction, five of which were identified as the S. coelicolor homologs of SpoIIIJ, Jag, GidB, Soj and SpoOJ in Bacillus subtilis and which have been ascribed different functions in this and other bacteria for either DNA replication, chromosomal partitioning or morphological development. The arrangement of the genes coding for the above five proteins in the trxA/B-rnpA region in S. coelicolor resembles that in Mycobacterium leprae, Mycobacterium tuberculosis, B. subtilis and Pseudomonas putida, and supports the view that many of the genes necessary for development and cell division in bacteria are organized in a similar fashion. In B. subtilis and P. putida, however, the trxA/B genes are not present in the above gene arrangement.
在天蓝色链霉菌染色体的trxA/B - rnpA区域确定了基因组织，该区域靠近复制起点oriC。此前，我们发现分别编码硫氧还蛋白和硫氧还蛋白还原酶的trxA和trxB基因在天蓝色链霉菌中作为一个基因簇存在，并包含在携带oriC和几个参与DNA复制的基因的黏粒H24上。在此我们表明trxA/B基因座位于距oriC约9.4kb处，给出trxA/B - rnpA区域的核苷酸序列，并利用序列分析来确定中间基因的性质。发现了7个开放阅读框，它们都朝同一方向，其中5个被鉴定为枯草芽孢杆菌中SpoIIIJ、Jag、GidB、Soj和SpoOJ的天蓝色链霉菌同源物，并且在该细菌和其他细菌中这些同源物已被赋予了DNA复制、染色体分配或形态发育等不同功能。天蓝色链霉菌trxA/B - rnpA区域中编码上述5种蛋白质的基因排列类似于麻风分枝杆菌、结核分枝杆菌、枯草芽孢杆菌和恶臭假单胞菌中的排列，这支持了细菌中许多发育和细胞分裂所需基因以类似方式组织的观点。然而，在枯草芽孢杆菌和恶臭假单胞菌中，trxA/B基因并不以上述基因排列存在。
