Calcutt M J
Department of Biochemistry, University of Missouri-Columbia 65212.
Gene. 1994 Dec 30;151(1-2):23-8. doi: 10.1016/0378-1119(94)90628-9.
The gene organization has been determined for an 8-kb portion of the Streptomyces coelicolor chromosome close to the origin of DNA replication (oriC). Hybridization and DNA sequence analyses revealed the presence of five open reading frames (ORFs) oriented in the same direction as the proximal dnaA and dnaN genes. The deduced products of three of the ORFs have been identified as the S. coelicolor homologs of RecF, GyrB and GyrA. These genes are usually clustered in the dnaA region of bacterial chromosomes. In S. coelicolor however, the usual gene arrangement is altered. The recF gene is flanked by two ORFs, one of which encodes a protein with significant similarity to 6-phosphogluconate dehydrogenases (6PGDH), an enzyme that is not immediately linked to DNA metabolism.
已确定了天蓝色链霉菌染色体中靠近DNA复制起点（oriC）的一个8 kb区域的基因组织。杂交和DNA序列分析表明，存在5个与近端dnaA和dnaN基因方向相同的开放阅读框（ORF）。其中3个ORF的推导产物已被鉴定为RecF、GyrB和GyrA的天蓝色链霉菌同源物。这些基因通常聚集在细菌染色体的dnaA区域。然而，在天蓝色链霉菌中，通常的基因排列发生了改变。recF基因两侧是两个ORF，其中一个编码与6-磷酸葡萄糖酸脱氢酶（6PGDH）具有显著相似性的蛋白质，该酶与DNA代谢并无直接关联。
