Wang G L, Song W Y, Ruan D L, Sideris S, Ronald P C
Department of Plant Pathology, University of California, Davis 95616, USA.
Mol Plant Microbe Interact. 1996 Dec;9(9):850-5. doi: 10.1094/mpmi-9-0850.
The cloned rice gene, Xa21, confers resistance to multiple pathogen isolates of Xanthomonas oryzae pv. oryzae in transgenic plants. The resistance phenotype was stably transmitted to T1 progeny and inherited as a single locus. The T1 progeny were tested for resistance to 32 X. oryzae pv. oryzae isolates from eight countries. Both the engineered line and the donor line showed resistance to 29 isolates and susceptibility to three isolates. The identical resistance spectrum of both lines indicates that the presence of a single member of a multigene family, Xa21, is sufficient to confer multi-isolate resistance. The results presented here have important implications for engineering disease resistance in crop plants.
克隆的水稻基因Xa21使转基因植物对水稻白叶枯病菌（Xanthomonas oryzae pv. oryzae）的多个病原菌分离株具有抗性。这种抗性表型稳定地传递给T1代子代，并作为单一位点遗传。对T1代子代进行了对来自8个国家的32个水稻白叶枯病菌分离株的抗性测试。工程株系和供体系对29个分离株表现出抗性，对3个分离株表现出敏感性。两个株系相同的抗性谱表明，多基因家族的单个成员Xa21的存在足以赋予多分离株抗性。本文给出的结果对作物抗病性工程具有重要意义。
