Song W Y, Wang G L, Chen L L, Kim H S, Pi L Y, Holsten T, Gardner J, Wang B, Zhai W X, Zhu L H, Fauquet C, Ronald P
Department of Plant Pathology, University of California, Davis 95616, USA.
Science. 1995 Dec 15;270(5243):1804-6. doi: 10.1126/science.270.5243.1804.
The rice Xa21 gene, which confers resistance to Xanthomonas oryzae pv. oryzae race 6, was isolated by positional cloning. Fifty transgenic rice plants carrying the cloned Xa21 gene display high levels of resistance to the pathogen. The sequence of the predicted protein, which carries both a leucine-rich repeat motif and a serine-threonine kinase-like domain, suggests a role in cell surface recognition of a pathogen ligand and subsequent activation of an intracellular defense response. Characterization of Xa21 should facilitate understanding of plant disease resistance and lead to engineered resistance in rice.
通过图位克隆分离出了赋予水稻对稻白叶枯病菌6号生理小种抗性的Xa21基因。五十株携带克隆的Xa21基因的转基因水稻植株对该病原菌表现出高水平抗性。预测蛋白质的序列含有富含亮氨酸的重复基序和丝氨酸 - 苏氨酸激酶样结构域，这表明其在细胞表面识别病原菌配体以及随后激活细胞内防御反应中发挥作用。对Xa21的特性分析应有助于理解植物抗病性，并在水稻中实现工程化抗性。
