Nakagawa S, Toritsuka Y, Wakitani S, Denno K, Tomita T, Owaki H, Kimura T, Shino K, Ochi T
Department of Orthopaedic Surgery, Osaka University Medical School, Japan.
J Rheumatol. 1996 Dec;23(12):2098-103.
To observe the migration of bone marrow stromal cells into the joint cavity and the contribution of such cells to synovial proliferation in rats with collagen induced arthritis (CIA).
After bone marrow stromal cells preliminarily labeled with fluorescent dye or 3H thymidine accumulated in the bone marrow of splenectomized rats by intraperitoneal injection, the migration of labeled stromal cells in rats with CIA was analyzed by liquid scintillation counting, autoradiography, and fluorography.
In splenectomized control rats, labeled bone marrow stromal cells were mostly found in the bone marrow and not in the synovium. Over 2 weeks after immunization, labeled stromal cells were microscopically found migrating directly into the joint cavity through the area between the articular margin and the synovial insertion (the bare zone). Labeled stromal cells were mainly found in the sublining layers of proliferating synovial tissue.
At the onset of CIA, bone marrow stromal cells migrated from the bone marrow into the affected joint cavity and seemed to contribute to synovial proliferation.
观察胶原诱导性关节炎（CIA）大鼠骨髓基质细胞向关节腔的迁移以及这些细胞对滑膜增殖的作用。
通过腹腔注射将预先用荧光染料或³H胸腺嘧啶核苷标记的骨髓基质细胞聚集在脾切除大鼠的骨髓中后，采用液体闪烁计数法、放射自显影法和荧光显影法分析CIA大鼠中标记的基质细胞的迁移情况。
在脾切除的对照大鼠中，标记的骨髓基质细胞大多存在于骨髓中，而非滑膜中。免疫后2周以上，显微镜下发现标记的基质细胞通过关节边缘与滑膜附着处之间的区域（裸区）直接迁移到关节腔。标记的基质细胞主要存在于增殖滑膜组织的衬里下层。
在CIA发病时，骨髓基质细胞从骨髓迁移到受影响的关节腔，似乎对滑膜增殖有作用。
