Escamilla L, Mancilla R, Glender W, López-Marín L M
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Circuito Interior, México.
Am J Respir Crit Care Med. 1996 Dec;154(6 Pt 1):1864-7. doi: 10.1164/ajrccm.154.6.8970382.
Glycolipids belonging to the family of acylated trehaloses were isolated from Mycobacterium fortuitum, a rapidly growing mycobacterial species, and tested in the serologic diagnosis of human pulmonary tuberculosis by enzyme-linked immunosorbent assay. Di- and tri-O-acylated trehaloses from M. fortuitum reacted with serum antibodies of patients with pulmonary tuberculosis at higher titers than did with sera from healthy donors. With both glycolipids, the sensitivity of the test was above 0.80 at a chosen specificity of 0.98. Individuals with treated tuberculosis showed lower antibody titers compared with their initial reactivities. These data show that M. fortuitum could be used as a surrogate source of antigens for tuberculosis serodiagnosis.
从快速生长的分枝杆菌——偶然分枝杆菌中分离出属于酰化海藻糖家族的糖脂，并通过酶联免疫吸附测定法对其在人类肺结核血清学诊断中的应用进行了测试。来自偶然分枝杆菌的二 - O - 酰化海藻糖和三 - O - 酰化海藻糖与肺结核患者血清抗体反应的滴度高于与健康供体血清反应的滴度。对于这两种糖脂，在选定特异性为0.98时，检测灵敏度高于0.80。与初始反应性相比，接受过治疗的结核病患者抗体滴度较低。这些数据表明，偶然分枝杆菌可用作肺结核血清学诊断的替代抗原来源。
