[结核分枝杆菌两种糖脂在结核病血清诊断中的评估]
[Evaluation of two glycolipids from M. tuberculosis in the serodiagnosis of tuberculosis].
作者信息
Hou W, Wu Q, Zhang P
机构信息
Institute of Dermatology, CAMS, Nanjing.
出版信息
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1994 Oct;16(5):374-7.
Sera from 112 healthy controls and 120 pulmonary tuberculous patients (61 untreated patients and 59 active patients) were assayed, by ELISA, to test the activity of IgG and IgM antibodies against antigen of 2, 3-diacyl-trehalose-2'-sulphate (SL IV) a phenolglycolipid antigen (PGLTb1) and purified protein derivative (PPD) from M. tuberculosis. Respectively, for SL IV-IgG-ELISA, SL IV-IgM-ELISA, PGLTb1-IgG-ELISA, PGLTb1-IgM-ELISA, PPD-IgG-ELISA, the specificities were of 96.43, 96.43, 96.43, 96.43 and 95.53%; the sensitivities were of 51.67, 32.50, 14.17, 18.33 and 33.33%; the efficiencies were of 73.28, 63.36, 53.88, 56.03 and 62.93%; the predictive values for a positive result were of 96.87, 86.67, 80.95, 84.62 and 88.64%. Among the three antigens tested, SL IV was found to be better than PGLTb1 and PPD.
采用酶联免疫吸附测定法（ELISA）检测了112名健康对照者以及120例肺结核患者（61例未经治疗患者和59例活动期患者）的血清，以检测针对2,3 - 二酰基海藻糖 - 2'- 硫酸盐（SL IV）、一种酚糖脂抗原（PGLTb1）和结核分枝杆菌纯化蛋白衍生物（PPD）抗原的IgG和IgM抗体活性。对于SL IV - IgG - ELISA、SL IV - IgM - ELISA、PGLTb1 - IgG - ELISA、PGLTb1 - IgM - ELISA、PPD - IgG - ELISA，特异性分别为96.43%、96.43%、96.43%、96.43%和95.53%；敏感性分别为51.67%、32.50%、14.17%、18.33%和33.33%；效率分别为73.28%、63.36%、53.88%、56.03%和62.93%；阳性结果预测值分别为96.87%、86.67%、80.95%、84.62%和88.64%。在所检测的三种抗原中，发现SL IV优于PGLTb1和PPD。
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