Muñoz M, Lanéelle M A, Luquin M, Torrelles J, Julián E, Ausina V, Daffé M
Institut de Pharmacologie et de Biologie Structurale du CNRS, Toulouse, France.
FEMS Microbiol Lett. 1997 Dec 15;157(2):251-9. doi: 10.1111/j.1574-6968.1997.tb12781.x.
A careful re-examination of the glycolipid content of clinical isolates and reference strains of the tubercle bacillus, Mycobacterium tuberculosis, led to the identification of a glycoconjugate that passed unnoticed in earlier studies. Nuclear magnetic resonance spectroscopy, gas chromatography-mass spectrometry and chemical degradations were used to identify the glycolipid as a 2,3,6-triacyl trehalose. The glycolipid contains a phthienoic acyl substituent, a family of multimethyl-branched, alpha,beta-unsaturated fatty acids specific for virulent strains of the tubercle bacillus. It reacted with sera from tuberculosis patients with a specificity and sensitivity of 96.2% and 76%, respectively. Comparable data were obtained with the 2,3-diacyl trehaloses of M. tuberculosis and M. fortuitum and with the triacyl trehaloses of M. fortuitum, suggesting that the antigens from the latter species may be used for the serodiagnosis of tuberculosis.
对结核分枝杆菌临床分离株和参考菌株的糖脂含量进行仔细的重新检查，发现了一种在早期研究中未被注意到的糖缀合物。利用核磁共振光谱、气相色谱 - 质谱联用和化学降解方法，将该糖脂鉴定为2,3,6 - 三酰海藻糖。该糖脂含有一个吩噻嗪酰基取代基，这是一类多甲基支链的α,β - 不饱和脂肪酸，是结核分枝杆菌毒力菌株所特有的。它与结核病患者血清反应，特异性和敏感性分别为96.2%和76%。用结核分枝杆菌和偶然分枝杆菌的2,3 - 二酰海藻糖以及偶然分枝杆菌的三酰海藻糖获得了类似的数据，这表明后一种菌的抗原可用于结核病的血清学诊断。
