Fiedler M A, Wernke-Dollries K, Stark J M
Department of Pediatrics, Children's Hospital Research Foundation, Cincinnati, Ohio 45229-3039, USA.
J Virol. 1996 Dec;70(12):9079-82. doi: 10.1128/JVI.70.12.9079-9082.1996.
Previous studies have demonstrated that respiratory syncytial virus (RSV) infection of airway epithelial cells results in the expression of a number of cytokines, such as interleukin-8 (IL-8), that are transcriptionally regulated by nuclear factor kappaB (NF-kappaB). In the studies reported here, we demonstrate that treatment of RSV-infected A549 cells with 100 microg of ribavirin (a viral replication inhibitor) per ml results in reversal of RSV-induced NF-kappaB activation, IL-8 mRNA expression, and IL-8 protein production in A549 cells. These data confirm that viral replication is a key step in RSV-induced NF-kappaB activation and IL-8 production.
先前的研究表明，呼吸道合胞病毒（RSV）感染气道上皮细胞会导致多种细胞因子的表达，如白细胞介素-8（IL-8），这些细胞因子受核因子κB（NF-κB）的转录调控。在本报告的研究中，我们证明，用每毫升100微克利巴韦林（一种病毒复制抑制剂）处理受RSV感染的A549细胞，可使A549细胞中RSV诱导的NF-κB激活、IL-8 mRNA表达和IL-8蛋白产生逆转。这些数据证实，病毒复制是RSV诱导NF-κB激活和IL-8产生的关键步骤。
