Isogai E, Isogai H, Kimura K, Hayashi S, Kubota T, Nishikawa T, Nakane A, Fujii N
Department of Preventive Dentistry, Health Sciences University of Hokkaido, Japan.
Microb Pathog. 1996 Dec;21(6):413-9. doi: 10.1006/mpat.1996.0072.
This study describes the detection of cytokines, interleukin-1 alpha (IL-1 alpha), tumor necrosis-factor alpha (TNF alpha) and interleukin-6 (IL-6) in the serum and brain homogenates of susceptible and resistant strains of mice infected with distinct species/strains of Borrelia. Significant elevation of inflammatory cytokines was found in the sera of susceptible C3H/ HeN mice but not in the sera of resistant BALB/c mice on days 7 and 45 after inoculation. Brain cytokines were observed in C3H/HeN mice infected with B. garinii and B. afzelii and the production was associated with the presence of spirochetes. B. japonica was the only exception in disease-susceptibility and cytokine production of mice. These results suggest that there are differences in pathogenic potential amongst Borrelia strains, and that the susceptible mice demonstrate a stronger cytokine response and greater frequency of recovery of Borrelia from the brain than do the resistant mice.
本研究描述了在感染不同种/株疏螺旋体的易感和抗性小鼠品系的血清及脑匀浆中检测细胞因子白细胞介素-1α（IL-1α）、肿瘤坏死因子α（TNFα）和白细胞介素-6（IL-6）的情况。接种后第7天和第45天，在易感的C3H/HeN小鼠血清中发现炎性细胞因子显著升高，而在抗性BALB/c小鼠血清中未发现。在感染了伽氏疏螺旋体和阿氏疏螺旋体的C3H/HeN小鼠中观察到脑细胞因子，其产生与螺旋体的存在有关。日本疏螺旋体在小鼠的疾病易感性和细胞因子产生方面是唯一的例外。这些结果表明，疏螺旋体菌株之间的致病潜力存在差异，并且易感小鼠比抗性小鼠表现出更强的细胞因子反应以及从脑中回收疏螺旋体的频率更高。
