Wallace J S, Jones K
Division of Biological Sciences, IEBS, Lancaster University, UK.
J Appl Bacteriol. 1996 Dec;81(6):663-8. doi: 10.1111/j.1365-2672.1996.tb03562.x.
The use of selective and differential agars for the isolation of Escherichia coli O157 from the faeces of dairy herds was investigated. Out of the 614 isolates which were positive for one or more of the selective criteria employed in the media only four proved to be E. coli O157. Ninety-nine per cent of the isolates were false positives. The procedure which resulted in the isolation of E. coli O157 from faecal samples was enrichment in modified Tryptone Soya Broth supplemented with novobiocin and subsequent growth on Chromagar O157.
对使用选择性和鉴别性琼脂从奶牛粪便中分离大肠杆菌O157进行了研究。在所检测的614株分离菌中，仅4株符合培养基中一种或多种选择标准的菌株被证实为大肠杆菌O157，其余99%的分离菌均为假阳性。从粪便样本中分离大肠杆菌O157的方法是，先在添加了新生霉素的改良胰蛋白胨大豆肉汤中富集培养，随后在O157显色培养基上培养。
