Di Nuzzo S, de Rie M A, van der Loos C M, Bos J D, Teunissen M B
Department of Dermatology, University of Amsterdam, The Netherlands.
Photochem Photobiol. 1996 Dec;64(6):988-93. doi: 10.1111/j.1751-1097.1996.tb01866.x.
The proportion and composition of the human cutaneous CD3+ T lymphocyte population was determined in situ following a single exposure to physiological, erythema-inducing doses of simulated solar radiation, mainly consisting of UV radiation. Biopsies were taken 1, 2 and 7 days after local irradiation of normal volunteers with 1, 2 and 4 MED by a xenonarc lamp and immunohistochemistry was performed on cryostat sections. Ultraviolet radiation caused an initial decrease of intraepidermal CD3+ T-cell numbers or even could lead to T-cell depletion 24 and 48 h postirradiation, and this was followed by an infiltration of T cells in the epidermis as determined 1 week after UV exposure. The number of dermal CD3+ T cells was increased 24 h after irradiation, reached a maximum at 48 h and subsequently declined at day 7, though remained significantly higher than the unirradiated control. Double staining demonstrated that the CD3+ T cells, which immigrated into the (epi)dermis upon UV exposure, coexpressed CD4 but not CD8. Therefore the CD4/CD8 ratio in skin was markedly increased during the first week upon UV exposure. Our time course study shows that UV radiation affects the T-cell population within human skin by depleting the majority of epidermal T cells and initiating a selective influx of CD4+ T cells.
单次暴露于主要由紫外线辐射构成的生理性红斑诱导剂量的模拟太阳辐射后，对人体皮肤CD3⁺ T淋巴细胞群体的比例和组成进行原位测定。用氙弧灯对正常志愿者进行1、2和4个最小红斑量（MED）的局部照射，在照射后1、2和7天取活检组织，并对冰冻切片进行免疫组织化学检测。紫外线辐射导致表皮内CD3⁺ T细胞数量最初减少，甚至在照射后24和48小时可导致T细胞耗竭，随后在紫外线暴露1周后确定表皮有T细胞浸润。真皮CD3⁺ T细胞数量在照射后24小时增加，在48小时达到峰值，随后在第7天下降，尽管仍显著高于未照射的对照组。双重染色显示，紫外线暴露后迁移到（表）皮的CD3⁺ T细胞共表达CD4但不表达CD8。因此，在紫外线暴露后的第一周内，皮肤中的CD4/CD8比值显著增加。我们的时间进程研究表明，紫外线辐射通过消耗大多数表皮T细胞并引发CD4⁺ T细胞的选择性流入来影响人体皮肤内的T细胞群体。
