• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

Clinical application of reverse transcription-polymerase chain reaction for HIV infection.

作者信息

Holodniy M

机构信息

Division of Infectious Diseases, Department of Veterans Affairs Medical Center, Palo Alto, California.

出版信息

Clin Lab Med. 1994 Jun;14(2):335-49.

PMID:7523019
Abstract

This article describes the current knowledge of the various RT-PCR assays and their clinical application in HIV disease. The importance of assay precision and sample handling is emphasized. A review of quantification techniques is also presented. Finally, the relevant literature describing the application of RT-PCR for both virion RNA in plasma and cellular mRNA is reviewed.

摘要

相似文献

1
Clinical application of reverse transcription-polymerase chain reaction for HIV infection.
Clin Lab Med. 1994 Jun;14(2):335-49.
2
Sensitive detection and quantification of particle-associated reverse transcriptase in plasma of HIV-1-infected individuals by the product-enhanced reverse transcriptase (PERT) assay.通过产物增强逆转录酶(PERT)测定法对HIV-1感染个体血浆中与颗粒相关的逆转录酶进行灵敏检测和定量分析。
J Med Virol. 1996 May;49(1):23-8. doi: 10.1002/(SICI)1096-9071(199605)49:1<23::AID-JMV4>3.0.CO;2-M.
3
Clinical comparison of branched DNA and reverse transcriptase-PCR and nucleic acid sequence-based amplification assay for the quantitation of circulating recombinant form_BC HIV-1 RNA in plasma.用于定量血浆中循环重组形式BC HIV-1 RNA的分支DNA、逆转录聚合酶链反应及基于核酸序列的扩增检测法的临床比较
AIDS. 2007 Dec;21 Suppl 8:S27-32. doi: 10.1097/01.aids.0000304693.16767.66.
4
TaqMan 5'-nuclease human immunodeficiency virus type 1 PCR assay with phage-packaged competitive internal control for high-throughput blood donor screening.用于高通量献血者筛查的带有噬菌体包装竞争性内对照的TaqMan 5'-核酸酶1型人类免疫缺陷病毒聚合酶链反应检测法
J Clin Microbiol. 2001 Dec;39(12):4302-8. doi: 10.1128/JCM.39.12.4302-4308.2001.
5
[HIV quantification--how and why].
Ugeskr Laeger. 1997 Dec 29;160(1):18-24.
6
A novel real-time multiplex reverse transcriptase-polymerase chain reaction for the detection of HIV-1 RNA by using dual-specific armored RNA as internal control.一种新型实时多重逆转录-聚合酶链反应,用于通过使用双特异性装甲RNA作为内部对照来检测HIV-1 RNA。
Intervirology. 2008;51(1):42-9. doi: 10.1159/000119119. Epub 2008 Mar 3.
7
Fully automated quantification of human immunodeficiency virus (HIV) type 1 RNA in human plasma by the COBAS AmpliPrep/COBAS TaqMan system.使用COBAS AmpliPrep/COBAS TaqMan系统对人血浆中的1型人类免疫缺陷病毒(HIV)RNA进行全自动定量分析。
J Clin Virol. 2007 Apr;38(4):304-12. doi: 10.1016/j.jcv.2006.12.022. Epub 2007 Feb 27.
8
Comparative evaluation of the Cobas Amplicor HIV-1 Monitor Ultrasensitive Test, the new Cobas AmpliPrep/Cobas Amplicor HIV-1 Monitor Ultrasensitive Test and the Versant HIV RNA 3.0 assays for quantitation of HIV-1 RNA in plasma samples.对Cobas Amplicor HIV-1监测超敏检测、新型Cobas AmpliPrep/Cobas Amplicor HIV-1监测超敏检测以及Versant HIV RNA 3.0检测法在血浆样本中定量检测HIV-1 RNA的比较评估。
J Clin Virol. 2005 May;33(1):43-51. doi: 10.1016/j.jcv.2004.09.025.
9
Comparison of 3 nucleic acid isolation methods for the quantification of HIV-1 RNA by Cobas Taqman real-time polymerase chain reaction system.通过Cobas Taqman实时聚合酶链反应系统比较3种核酸提取方法用于定量HIV-1 RNA
Diagn Microbiol Infect Dis. 2009 Apr;63(4):365-71. doi: 10.1016/j.diagmicrobio.2008.12.014. Epub 2009 Feb 18.
10
Comparative evaluation of the VERSANT HIV-1 RNA 1.0 kinetic PCR molecular system (kPCR) for the quantification of HIV-1 plasma viral load.用于定量检测HIV-1血浆病毒载量的VERSANT HIV-1 RNA 1.0动力学PCR分子系统(kPCR)的比较评估。
J Clin Virol. 2009 Apr;44(4):297-301. doi: 10.1016/j.jcv.2009.01.004. Epub 2009 Feb 18.

引用本文的文献

1
A high-throughput gene expression analysis technique using competitive PCR and matrix-assisted laser desorption ionization time-of-flight MS.一种使用竞争性聚合酶链反应和基质辅助激光解吸电离飞行时间质谱的高通量基因表达分析技术。
Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3059-64. doi: 10.1073/pnas.0630494100. Epub 2003 Mar 6.
2
Transcription of genes encoding iron and heme acquisition proteins of Haemophilus influenzae during acute otitis media.流感嗜血杆菌铁和血红素获取蛋白编码基因在急性中耳炎期间的转录
Infect Immun. 1997 Nov;65(11):4696-700. doi: 10.1128/iai.65.11.4696-4700.1997.
3
A sensitive PCR assay system for the quantitation of viral genome equivalents: hepatitis C virus (HCV).
Arch Virol. 1996;141(11):2103-14. doi: 10.1007/BF01718218.