冰草花叶病毒和大麦花叶病毒部分基因组的分子克隆与核苷酸测序，这两种病毒是分类学上的马铃薯Y病毒科大麦病毒属的成员。

Molecular cloning and nucleotide sequencing of the partial genomes of Agropyron and Hordeum mosaic viruses, two members of the Rymovirus genus in the taxonomic family Potyviridae.

作者信息

Salm S N, Rey M E, Robertson N L, French R, Rabenstein F, Schubert J

机构信息

Range and Forage Institute, Pretoria, South Africa.

出版信息

Arch Virol. 1996;141(11):2115-27. doi: 10.1007/BF01718219.

DOI:10.1007/BF01718219

PMID:8973527

Abstract

Complementary DNAs encompassing the coat protein coding and adjacent regions of Agropyron mosaic virus (AgMV) and Hordeum mosaic virus (HoMV) were cloned and sequenced. Comparison with other sequenced potyviruses indicated that each clone contained the 3'-non-coding region (3'NCR), the coat protein (CP) gene and part of the nuclear inclusion protein (NIb) gene. Nucleotide and amino acid sequence comparisons of the 3'-terminal regions of these and other rymoviruses indicate that distinct groups exist. Ryegrass mosaic virus (RGMV) strains share sequence similarity with AgMV and HoMV. Wheat streak mosaic virus (WSMV) and brome streak mosaic virus (BrSMV) form a separate group, sharing limited sequence similarity with the other rymoviruses. It is proposed that subgroups occur within the Rymovirus genus, depending on the vector species involved in transmission and on sequences.

摘要

克隆并测序了包含冰草花叶病毒（AgMV）和大麦花叶病毒（HoMV）外壳蛋白编码区及相邻区域的互补DNA。与其他已测序的马铃薯Y病毒进行比较表明，每个克隆都包含3'-非编码区（3'NCR）、外壳蛋白（CP）基因和部分核内含蛋白（NIb）基因。对这些病毒及其他黑麦草花叶病毒3'-末端区域的核苷酸和氨基酸序列比较表明，存在不同的组。黑麦草花叶病毒（RGMV）毒株与AgMV和HoMV具有序列相似性。小麦线条花叶病毒（WSMV）和雀麦线条花叶病毒（BrSMV）形成一个单独的组，与其他黑麦草花叶病毒的序列相似性有限。有人提出，根据传播所涉及的介体种类和序列，黑麦草花叶病毒属内存在亚组。