Mathew J, Buchardt O
Center for Medical Biotechnology, H.C. Orsted Institute, University of Copenhagen, Denmark.
Bioconjug Chem. 1995 Sep-Oct;6(5):524-8. doi: 10.1021/bc00035a004.
Polymers capable of recognizing adenine in aqueous medium were developed by the molecular imprinting technique using methacrylic acid and 4(5)-vinylimidazole as comonomers with ethylene glycol dimethacrylate as the cross-linking agent under different polymerization conditions. The affinity of these polymers for adenine and other nucleotide bases was compared. The association constant for the binding of adenine to the polymer is calculated to be 4.3 x 10(3)M(-1). Furthermore, binding of adenosine 5'-triphosphate (ATP) to the polymers was evaluated, and an enhanced binding compared to adenine was observed. The binding of ATP is pH dependent, with the maximum around pH 3. Results have been explained based on the hydrogen bonding and ionic interactions between ATP and the ligands on the polymer matrix. The catalytic effect of these polymers for the hydrolysis of ATP is briefly discussed.
通过分子印迹技术，以甲基丙烯酸和4(5)-乙烯基咪唑作为共聚单体，乙二醇二甲基丙烯酸酯作为交联剂，在不同聚合条件下制备了能够在水性介质中识别腺嘌呤的聚合物。比较了这些聚合物对腺嘌呤和其他核苷酸碱基的亲和力。计算得出腺嘌呤与该聚合物结合的缔合常数为4.3×10³M⁻¹。此外，评估了三磷酸腺苷（ATP）与聚合物的结合情况，观察到与腺嘌呤相比，其结合增强。ATP的结合具有pH依赖性，在pH约为3时达到最大值。基于ATP与聚合物基质上配体之间的氢键和离子相互作用对结果进行了解释。简要讨论了这些聚合物对ATP水解的催化作用。
