Samal' A B, Koliadko M G, Sogrina E N, Iurkshtovich T L, Sidorenko G I
Eksp Klin Farmakol. 1996 Mar-Apr;59(2):27-30.
The effect of oxidized starch (OS) which contained 15% of COOH groups and its nitroether (NOS) with 4% of nitrogen on coagulation properties of rat blood was studied in vitro and in vivo. The results of the study in vitro showed that OS did not affect the function of the coagulation system. In contrast to OS, a dose-dependent increase in prothrombin-, thrombin time, and activated partial thromboplastin time was observed for NOS. The activity of the components of the internal coagulation pathway changed when the NOS concentration reached 0.1 mg/ml. At a concentration of 0.6 mg/ml and higher this compound affect the external pathway and final stage of coagulation. According to the efficiency (in vitro) of the influence on the thrombine time I mg/ml NOS corresponded to 0.2 U/ml of heparine. The anticoagulant effect of NOS was also observed in vivo along with reliable changes in thromboplastin and thrombin time. Antithrombin activity of plasma remained the same. Standard test was negative and indicated to the absence of fibrin monomers. The pronounced anticoagulant effect of NOS in the experiments in vitro and quick response in the experiments in vivo make it possible to consider this compound as anticoagulant of direct action.
研究了含15%羧基的氧化淀粉（OS）及其含4%氮的硝基醚（NOS）对大鼠血液凝血特性的体内外影响。体外研究结果表明，OS不影响凝血系统功能。与OS相反，观察到NOS使凝血酶原时间、凝血酶时间和活化部分凝血活酶时间呈剂量依赖性增加。当NOS浓度达到0.1mg/ml时，内源性凝血途径各成分的活性发生变化。在浓度为0.6mg/ml及更高时，该化合物影响外源性途径和凝血的最终阶段。根据对凝血酶时间的影响效率（体外），1mg/ml的NOS相当于0.2U/ml的肝素。在体内也观察到了NOS的抗凝作用，同时凝血活酶和凝血酶时间发生了可靠的变化。血浆抗凝血酶活性保持不变。标准试验为阴性，表明不存在纤维蛋白单体。NOS在体外实验中具有显著的抗凝作用，在体内实验中反应迅速，这使得可以将该化合物视为直接作用的抗凝剂。
