Ibeas J I, Lozano I, Perdigones F, Jimenez J
Unidad de Genetica, Facultad de Ciencias, Universidad de Malaga, Campus Universitario de Teatinos, Spain.
Appl Environ Microbiol. 1996 Mar;62(3):998-1003. doi: 10.1128/aem.62.3.998-1003.1996.
Brettanomyces sp. and its ascosporogenous sexual state, Dekkera sp., have been well documented as spoilage microorganisms, usually associated with barrel-aged red wines. In this report, we describe the genetic characterization, on the basis of DNA content per cell, electrophoretic karyotyping, and mitochondrial DNA restriction patterns, of a Dekkera yeast strain isolated from sherries and of a number of other Brettanomyces and Dekkera strains. By using a genomic DNA fragment of the isolated Dekkera strain, we developed a two-step PCR method which directs the specific amplification of target DNA from this strain and from other Brettanomyces-Dekkera strains. The method efficiently amplified the target DNA from intact cells, obviating DNA isolation, and yielded a detection limit of fewer than 10 yeast cells in contaminated samples of sherry.
酒香酵母属及其产子囊孢子的有性态——德克酵母属，已被充分证明是腐败微生物，通常与桶陈红葡萄酒有关。在本报告中，我们基于每个细胞的DNA含量、脉冲场凝胶电泳核型分析以及线粒体DNA限制性酶切图谱，描述了从雪利酒中分离出的一株德克酵母菌株以及其他一些酒香酵母属和德克酵母属菌株的遗传特征。通过使用分离出的德克酵母菌株的基因组DNA片段，我们开发了一种两步PCR方法，该方法可从该菌株以及其他酒香酵母 - 德克酵母属菌株中特异性扩增目标DNA。该方法能有效地从完整细胞中扩增目标DNA，无需进行DNA分离，并且在受污染的雪利酒样品中对少于10个酵母细胞的检测限。
