Canli M, Stagg R M
Department of Zoology, University of Glasgow, United Kingdom.
Arch Environ Contam Toxicol. 1996 Nov;31(4):494-501. doi: 10.1007/BF00212433.
Norway lobsters, Nephrops norvegicus were exposed for 18 days to combinations of cadmium, copper and zinc at three sublethal concentrations (1, 5, 25 micrograms/L for Cd and Cu, and 8, 40 and 200 micrograms/L for Zn). Male animals were exposed to all three concentrations, while female animals were studied only in one (highest) concentration of the metals. Activities of Na,K-ATPase and both oligomycin-sensitive and insensitive Mg-ATPase were investigated in relation to metal exposure. A group of male Nephrops of different size groups from a single location in the Clyde Sea was also sampled to investigate the relationship between size and ATPase activity. Cadmium concentrations increased significantly with increases in exposure concentrations in both male and female animals, whereas copper and zinc increased significantly only in the highest treatment in males. There was no significant difference in concentrations of the metals in control male and female animals, whereas males accumulated significantly higher levels of copper and zinc in the highest concentrations of the metals. Control males showed higher activity of Na,K-ATPase than control females. Na,K-ATPase activity was significantly inhibited in male animals exposed to metals whereas in female animals there was only a significant inhibition in total Mg-ATPase activity. Na,K-ATPase activity had a negative relationship with copper in males, while in treated females this ATPase had positive relationship with zinc and copper. There were negative relationships between the activities of Na,K-ATPase and oligomycin sensitive Mg-ATPase with carapace length of the animals.
将挪威龙虾（Nephrops norvegicus）暴露于镉、铜和锌的组合环境中18天，这三种金属设置了三个亚致死浓度（镉和铜为1、5、25微克/升，锌为8、40和200微克/升）。雄性动物暴露于所有三种浓度环境中，而雌性动物仅在一种（最高）金属浓度下进行研究。研究了与金属暴露相关的钠钾ATP酶以及对寡霉素敏感和不敏感的镁ATP酶的活性。还从克莱德海的一个地点采集了一组不同大小组的雄性挪威龙虾样本，以研究大小与ATP酶活性之间的关系。在雄性和雌性动物中，镉浓度均随暴露浓度的增加而显著增加，而铜和锌仅在雄性动物的最高处理浓度下显著增加。对照雄性和雌性动物体内金属浓度无显著差异，而在最高金属浓度下，雄性动物积累的铜和锌水平显著更高。对照雄性的钠钾ATP酶活性高于对照雌性。暴露于金属的雄性动物中钠钾ATP酶活性显著受到抑制，而在雌性动物中，仅总镁ATP酶活性受到显著抑制。雄性动物中钠钾ATP酶活性与铜呈负相关，而在经处理的雌性动物中，该ATP酶与锌和铜呈正相关。钠钾ATP酶和对寡霉素敏感的镁ATP酶的活性与动物的甲壳长度呈负相关。
