Watson C F, Benson W H
Division of Pharmacology and Toxicology, School of Pharmacy, Northeast Louisiana University, Monroe 71209-0470.
Ecotoxicol Environ Saf. 1987 Dec;14(3):252-9. doi: 10.1016/0147-6513(87)90068-6.
The comparative activity of gill ATPase was examined in the bluegill sunfish (Lepomis macrochirus), fathead minnow (Pimephales promelas), and golden shiner (Notemigonus crysoleucas). Basal Na/K ATPase activity was highest in bluegill sunfish (1.46 mumol Pi/mg protein/hr) and lowest in golden shiners (1.01 mumol Pi/mg protein/hr). While a stimulation of Na/K ATPase activity was observed at an exposure concentration of 1 micrograms Cd/liter in the bluegill sunfish and fathead minnows, an inhibition of enzymatic activity was observed at higher exposure concentrations (10 and 100 micrograms Cd/liter). Gill Na/K ATPase activity in golden shiners was not significantly influenced by cadmium exposure. The observed insensitivity of Na/K ATPase in golden shiners may, in part, be related to high background concentrations of cadmium in gill tissue. In all three species examined, gill residual ATPase activity was not significantly altered by cadmium exposure.
对蓝鳃太阳鱼（Lepomis macrochirus）、黑头呆鱼（Pimephales promelas）和金色闪光鱼（Notemigonus crysoleucas）的鳃ATP酶活性进行了比较研究。基础钠钾ATP酶活性在蓝鳃太阳鱼中最高（1.46微摩尔无机磷/毫克蛋白质/小时），在金色闪光鱼中最低（1.01微摩尔无机磷/毫克蛋白质/小时）。在蓝鳃太阳鱼和黑头呆鱼中，当暴露浓度为1微克镉/升时，钠钾ATP酶活性受到刺激，但在较高暴露浓度（10和100微克镉/升）下，酶活性受到抑制。金色闪光鱼的鳃钠钾ATP酶活性不受镉暴露的显著影响。观察到金色闪光鱼中钠钾ATP酶的不敏感性可能部分与鳃组织中镉的高背景浓度有关。在所研究的所有三个物种中，镉暴露并未显著改变鳃残余ATP酶活性。
