Erickson D R, Ordille S, Martin A, Bhavanandan V P
Department of Surgery (Division of Urology), Milton S. Hershey Medical Center, Pennsylvania State University College of Medicine, Hershey, USA.
J Urol. 1997 Jan;157(1):61-4.
We compared urinary glycosaminoglycan levels in patients with interstitial cystitis and healthy controls.
Total sulfated glycosaminoglycans assayed by dimethylmethylene blue binding and individual glycosaminoglycans analyzed by cellulose acetate electrophoresis were compared in patients with interstitial cystitis and healthy controls. Also, multiple urine samples were obtained from healthy female controls for 2 months to assess the relationship of urinary glycosaminoglycan and creatinine concentrations, and to determine whether glycosaminoglycan excretion changes during the menstrual cycle.
Total sulfated glycosaminoglycan and creatinine concentrations correlated well in random voided samples. Menstrual cycle day did not affect total sulfated glycosaminoglycan levels. Cellulose acetate electrophoresis revealed 3 bands corresponding to chondroitin sulfates, heparan sulfate and acidic glycoprotein. Patients with interstitial cystitis had decreased urinary concentrations of each of these individual components and total sulfated glycosaminoglycans. However, glycosaminoglycan-to-creatinine ratios were similar in interstitial cystitis and control urine.
Using these assays total and individual urinary glycosaminoglycan levels normalized to creatinine were not altered in interstitial cystitis.
我们比较了间质性膀胱炎患者与健康对照者的尿糖胺聚糖水平。
对间质性膀胱炎患者和健康对照者，比较通过二甲基亚甲蓝结合法测定的总硫酸化糖胺聚糖以及通过醋酸纤维素电泳分析的单个糖胺聚糖。此外，从健康女性对照者处获取多个尿液样本，为期2个月，以评估尿糖胺聚糖与肌酐浓度的关系，并确定月经周期中糖胺聚糖排泄量是否变化。
随机排尿样本中，总硫酸化糖胺聚糖与肌酐浓度相关性良好。月经周期日不影响总硫酸化糖胺聚糖水平。醋酸纤维素电泳显示出3条带，分别对应硫酸软骨素、硫酸乙酰肝素和酸性糖蛋白。间质性膀胱炎患者这些单个成分及总硫酸化糖胺聚糖的尿浓度均降低。然而，间质性膀胱炎患者尿液与对照尿液中的糖胺聚糖与肌酐比值相似。
采用这些检测方法，间质性膀胱炎患者中经肌酐标准化后的总尿糖胺聚糖水平及单个尿糖胺聚糖水平未发生改变。
