Creamer P, Hall N D
Rheumatology Unit, Bristol Royal Infirmary, United Kingdom.
Ann Rheum Dis. 1996 Nov;55(11):844-7. doi: 10.1136/ard.55.11.844.
To compare expression of the p75 chain of the interleukin-2 receptor (IL-2Rp75, CD122) on peripheral and synovial mononuclear cells in rheumatoid and non-rheumatoid inflammatory arthritis.
Peripheral blood (PBMC) and synovial (SFMC) mononuclear cells were isolated from subjects with rheumatoid arthritis (n = 16) and non-rheumatoid inflammatory arthritis (n = 12). PBMC were isolated from six healthy controls. Expression of CD122 was examined using indirect immunofluorescence and quantitative flow cytometry.
There was no difference in IL-2Rp75 expression on PBMC from rheumatoid arthritis patients, non-rheumatoid arthritis patients, and controls. In subjects with rheumatoid arthritis there was no difference in IL-2Rp75 expression on PBMC and SFMC. However, in the non-rheumatoid arthritis group there was an increase in IL-2Rp75 expression on SFMC compared with PBMC (P = 0.0032). On SFMC there was a greater expression of IL-2Rp75 in non-rheumatoid arthritis than in rheumatoid arthritis (P = 0.0007). Expression was greater on CD8 positive cells and in subjects with shorter duration of disease.
The p75 chain of the IL-2 receptor, an important T cell activation antigen, is not upregulated in synovial fluid. This appears to be a disease specific defect and provides further support for the concept of "frustrated" or incomplete T cell activation in this disease.
比较类风湿性和非类风湿性炎性关节炎患者外周血和滑膜单核细胞上白细胞介素-2受体（IL-2Rp75，CD122）p75链的表达情况。
从类风湿性关节炎患者（n = 16）和非类风湿性炎性关节炎患者（n = 12）中分离外周血单核细胞（PBMC）和滑膜单核细胞（SFMC）。从6名健康对照者中分离PBMC。采用间接免疫荧光和定量流式细胞术检测CD122的表达。
类风湿性关节炎患者、非类风湿性关节炎患者和对照者的PBMC上IL-2Rp75表达无差异。类风湿性关节炎患者的PBMC和SFMC上IL-2Rp75表达无差异。然而，在非类风湿性关节炎组中，SFMC上的IL-2Rp75表达较PBMC增加（P = 0.0032）。非类风湿性关节炎患者SFMC上的IL-2Rp75表达高于类风湿性关节炎患者（P = 0.0007）。CD8阳性细胞上的表达更高，且在病程较短的患者中表达更高。
IL-2受体的p75链，一种重要的T细胞活化抗原，在滑液中未上调。这似乎是一种疾病特异性缺陷，并为该疾病中“受阻”或不完全T细胞活化的概念提供了进一步支持。
