Goutaland C, Piens M A
Laboratoire de Parasitologie et de Pathologie Exotique, Domaine Universitaire Rockefeller, Lyon, France.
Pathol Biol (Paris). 1996 Oct;44(8):724-8.
107 Strains of different species of Candida have been tested by three methods of susceptibility to fluconazole. In addition, one reference strain has been used to verify three methods' reliability: one diffusion method on Shadomy's medium and two MIC liquid methods, on tube and on microtiter plate. Method on Shadomy's medium seems less sensitive for yeast susceptibility. Comparison of two liquid method of MIC show discordances on resistant strains (14% for method on tube and 26% for micromethod) By simplicity of execution and reliability in detection of natural resistances, micromethod of MIC seems the most convenient for yeasts determination in vitro to fluconazole.
已通过三种氟康唑敏感性测试方法对107株不同种类的念珠菌进行了检测。此外，还使用了一株参考菌株来验证这三种方法的可靠性：一种是在沙多米培养基上的扩散法，另外两种是MIC液体法，分别是试管法和微量滴定板法。沙多米培养基上的方法对酵母敏感性的检测似乎不太灵敏。两种MIC液体法的比较显示，在耐药菌株上存在不一致性（试管法为14%，微量法为26%）。就操作简便性和天然耐药性检测的可靠性而言，MIC微量法似乎是体外测定酵母对氟康唑敏感性最便捷的方法。
