Garmyn D, Monnet C, Martineau B, Guzzo J, Cavin J F, Diviès C
Laboratoire de Microbiologie, ENSBANA, Université de Bourgogne, Dijon, France.
FEMS Microbiol Lett. 1996 Dec 15;145(3):445-50. doi: 10.1111/j.1574-6968.1996.tb08614.x.
The alsD gene encoding alpha-acetolactate decarboxylase was isolated from a genomic library of Leuconostoc oenos, using a screening procedure developed on microtiter plates. The nucleotide sequence of alsD encodes a putative protein of 239 amino acids showing significant similarity with other bacterial alpha-acetolactate decarboxylases. Upstream from alsD lies an open reading frame (alsS) which is highly similar to bacterial genes coding for catabolic alpha-acetolactate synthases. Northern (RNA) blotting analyses indicated the presence of a 2.4-kb dicistronic transcript of alsS and alsD. This suggests that the alsS and alsD genes are organized in a single operon.
利用在微量滴定板上开发的筛选程序，从酒酒球菌的基因组文库中分离出编码α-乙酰乳酸脱羧酶的alsD基因。alsD的核苷酸序列编码一个由239个氨基酸组成的推定蛋白，该蛋白与其他细菌的α-乙酰乳酸脱羧酶具有显著的相似性。alsD上游存在一个开放阅读框（alsS），它与编码分解代谢型α-乙酰乳酸合酶的细菌基因高度相似。Northern（RNA）印迹分析表明存在一个2.4kb的alsS和alsD双顺反子转录本。这表明alsS和alsD基因组成一个单一的操纵子。
