Diderichsen B, Wedsted U, Hedegaard L, Jensen B R, Sjøholm C
Novo Nordisk, Bagsvaerd, Denmark.
J Bacteriol. 1990 Aug;172(8):4315-21. doi: 10.1128/jb.172.8.4315-4321.1990.
A gene for alpha-acetolactate decarboxylase (ALDC) was cloned from Bacillus brevis in Escherichia coli and in Bacillus subtilis. The 1.3-kilobase-pair nucleotide sequence of the gene, aldB, encoding ALDC and its flanking regions was determined. An open reading frame of 285 amino acids included a typical N-terminal signal peptide of 24 or 27 amino acids. A B. subtilis strain harboring the aldB gene on a recombinant plasmid processed and secreted ALDC. In contrast, a similar enzyme from Enterobacter aerogenes is intracellular.
从短短芽孢杆菌中克隆出α-乙酰乳酸脱羧酶（ALDC）基因，并在大肠杆菌和枯草芽孢杆菌中进行表达。测定了编码ALDC的aldB基因及其侧翼区域1.3千碱基对的核苷酸序列。一个由285个氨基酸组成的开放阅读框包含一个由24或27个氨基酸组成的典型N端信号肽。携带重组质粒上aldB基因的枯草芽孢杆菌菌株可加工并分泌ALDC。相比之下，产气肠杆菌中的一种类似酶存在于细胞内。
