Department of Chemical Technology, University of Calcutta, Kolkata, West Bengal 700 009, India.
J Anal Methods Chem. 2013;2013:351671. doi: 10.1155/2013/351671. Epub 2013 Nov 12.
The present work describes the isolation of α - and β -carotene from crude palm oil and their antioxidant potential in an in vitro model. Pure product was isolated by the method adopted. Antioxidant activities of the isolated α - and β -carotene were analyzed in five different concentrations of 0.001, 0.005, 0.01, 0.05, and 0.1% (w/v). From the several assays conducted, an observation was made that the antioxidant activity of the product shifted between antioxidant and prooxidant effects depending on the concentration and the system analyzed. The metal chelation, DPPH radical scavenging, and superoxide scavenging activities showed almost similar results in terms of high activity at lowest concentrations. ABTS-scavenging activity was displayed only by a particular antioxidant concentration of 0.1%. Lipid peroxidation assay pronounced the activity of 0.1% antioxidant in inhibiting oxidation of sensitive bioactive lipids. In vitro antidenaturation test again specified the efficacy of low concentrations in preventing protein denaturation. Through this study a definite dosage formulation for consumption of carotenoids is being proposed which will enhance health promotion and prevent chronic diseases when taken as fortified foods or dietary supplements.
本工作描述了从粗棕榈油中分离 α-和 β-胡萝卜素及其在体外模型中的抗氧化潜力。采用所采用的方法分离出纯产物。在 0.001、0.005、0.01、0.05 和 0.1%（w/v）的五个不同浓度下分析分离的 α-和 β-胡萝卜素的抗氧化活性。从进行的几项测定中观察到，产品的抗氧化活性根据浓度和分析的系统在抗氧化和促氧化作用之间发生变化。金属螯合、DPPH 自由基清除和超氧化物清除活性在最低浓度下表现出几乎相同的高活性。ABTS 清除活性仅由特定的 0.1%抗氧化剂浓度显示。脂质过氧化测定法阐明了 0.1%抗氧化剂抑制敏感生物活性脂质氧化的活性。体外抗变性试验再次指定了低浓度防止蛋白质变性的功效。通过这项研究，提出了一种确定的消费类胡萝卜素的剂量配方，当作为强化食品或膳食补充剂服用时，将增强健康促进作用并预防慢性疾病。
