Petroff O A, Ogino T, Alger J R
Department of Neurology, Yale University, New Haven, Connecticut 06510.
J Neurochem. 1988 Jul;51(1):163-71. doi: 10.1111/j.1471-4159.1988.tb04850.x.
The changes in 16 cerebral metabolites produced by cardiac arrest and subsequent room temperature autolysis were studied using high-resolution proton nuclear magnetic resonance spectroscopy. Biopsies of rabbit cerebral cortex, cerebral white matter, and cerebellum were quantitatively analyzed for acetate, alanine, gamma-aminobutyric acid, creatine, glutamate, glycine, inositol, lactate, N-acetylaspartate, phosphocreatine, succinate, taurine, and threonine. Of these, N-acetylaspartate and the total creatine pool are the best candidates for use as concentration reference standards linking in vitro to in vivo 1H nuclear magnetic resonance measurements. Both changed little immediately after death, and they varied in a distinctive way among cortex, white matter, and cerebellum.
采用高分辨率质子核磁共振波谱法研究了心脏骤停及随后室温下自溶过程中16种脑代谢物的变化。对兔大脑皮层、脑白质和小脑活检组织中的乙酸盐、丙氨酸、γ-氨基丁酸、肌酸、谷氨酸、甘氨酸、肌醇、乳酸、N-乙酰天门冬氨酸、磷酸肌酸、琥珀酸盐、牛磺酸和苏氨酸进行了定量分析。其中，N-乙酰天门冬氨酸和总肌酸池是用作将体外与体内1H核磁共振测量联系起来的浓度参考标准的最佳选择。两者在死亡后立即变化很小，并且在皮层、白质和小脑中以独特的方式变化。
