Yasui Y, Ohnishi O
Laboratory of Genetics, Faculty of Agriculture, Kyoto University, Japan.
Genes Genet Syst. 1996 Aug;71(4):219-24. doi: 10.1266/ggs.71.219.
Four species of Fagopyrum, two species of Persicaria and one species of Rumex were analyzed for the DNA sequences of their rbcL genes. In addition to these seven species, we analyzed the published or available sequence data of ten related taxa in Polygonales, Plumbaginales and Caryophyllales. By estimating the base substitution rate between each pair of species, and by constructing phylogenetic trees we concluded that: 1) In Fagopyrum, F. cymosum and F. tataricum are much more closely related than expected previously; this confirms the conclusion of Kishima et al. (1995). 2) The new classification of Fagopyrum species proposed by Ohnishi and Matsuoka (1996) based on morphology, isozyme and cpDNA variability is consistent with the rbcL sequences. 3) The Polygonales and the Plumbaginales should be separated from the Caryophyllales as concluded by Giannasi et al. (1992). 4) The separation of Fagopyrum from other genera in the Polygoneae has a solid molecular basis.
对四种荞麦属植物、两种蓼属植物和一种酸模属植物的rbcL基因的DNA序列进行了分析。除了这七个物种外，我们还分析了蓼目、蓝雪目和石竹目中十个相关分类群已发表的或可获得的序列数据。通过估计每对物种之间的碱基替换率，并构建系统发育树，我们得出以下结论：1）在荞麦属中，金荞麦和苦荞麦的亲缘关系比之前预期的更为密切；这证实了岸岛等人（1995年）的结论。2）大西和松冈（1996年）基于形态学、同工酶和叶绿体DNA变异性提出的荞麦属物种新分类与rbcL序列一致。3）正如贾纳西等人（1992年）所总结的，蓼目和蓝雪目应与石竹目分开。4）荞麦属与蓼科其他属的分离有坚实的分子基础。
