Craver R D, Abermanis J G, Warrier R P, Ode D L, Hempe J M
Department of Pathology, Louisiana State University, New Orleans, USA.
Am J Clin Pathol. 1997 Jan;107(1):88-91. doi: 10.1093/ajcp/107.1.88.
Simultaneously measuring major and minor hemoglobin (Hb) variants by capillary isoelective focusing, we obtained HbA2 intervals in healthy volunteers (n = 412) (reference value) and patients with HbS or beta-thalassemia. We classified normal HbA2 reference intervals into three age groups: 5 months or younger (1.2% +/- 1.5%), 6 months to 1 year (2.2% +/- 0.9%), and 1 year or older (2.4% +/- 0.9%). These intervals were comparable to those used with other methods. Patients 1 year of age or older with HbS had significantly higher HbA2 levels (sickle cell trait, 2.9% +/- 0.9%; sickle cell anemia, 2.8% +/- 1.0%; P < .05). Although reference HbA2 intervals overlapped those in patients with HbS, no overlap in HbA2 levels was noted between these groups and patients with beta-thalassemia (observed range, 4.3% to 7.5%). The higher than normal HbA2 interval in patients with HbS must be considered before a diagnosis of sickle cell trait or sickle cell disease with beta-thalassemia is made.
通过毛细管等电聚焦同时测量主要和次要血红蛋白（Hb）变体，我们获得了健康志愿者（n = 412）（参考值）以及患有血红蛋白S（HbS）或β地中海贫血患者的HbA2区间。我们将正常HbA2参考区间分为三个年龄组：5个月及以下（1.2%±1.5%）、6个月至1岁（2.2%±0.9%）和1岁及以上（2.4%±0.9%）。这些区间与其他方法所使用的区间相当。1岁及以上患有HbS的患者HbA2水平显著更高（镰状细胞性状，2.9%±0.9%；镰状细胞贫血，2.8%±1.0%；P <.05）。虽然参考HbA2区间与患有HbS的患者的区间有重叠，但在这些组与β地中海贫血患者之间未观察到HbA2水平有重叠（观察范围，4.3%至7.5%）。在诊断镰状细胞性状或合并β地中海贫血的镰状细胞病之前，必须考虑患有HbS的患者中高于正常的HbA2区间。
