Department of Biochemistry, University of Georgia, 30602, Athens, GA, USA.
Plant Mol Biol. 1988 May;11(3):277-91. doi: 10.1007/BF00027385.
Lea genes code for mRNAs and proteins that are late embryogenesis abundant in higher plant seed embryos. They appear to be ubiquitous in higher plants and may be induced to high levels of expression in other tissues and at other times of ontogeny by ABA and/or desiccation. Presented here are the genomic and cDNA sequences for 6 of these genes from cotton seed embryos and the derived amino acid sequences of the corresponding proteins.The Lea genes contain the standard sequence features of eucaryotic genes (TATA box and poly (A) addition sequences) and have 1 or more introns. Sequences differences between cDNA and genomic DNA confirm the existence of small multigene families for several Lea genes. The amino acid composition and sequence for the Lea proteins are unusual. Five are extremely hydrophilic, four contain no cys or trp and 4 have sequence domains that suggest amphiphilic helical structures. Hypothetical functions in desiccation survival, based on amino acid sequence, are discussed.
Lea 基因编码的 mRNA 和蛋白质在高等植物种子胚胎中晚期胚胎丰富。它们似乎在高等植物中普遍存在，并且可以通过 ABA 和/或干燥诱导在其他组织和其他发育时期高水平表达。本文介绍了来自棉花种子胚胎的 6 个这样的基因的基因组和 cDNA 序列，以及相应蛋白质的推导氨基酸序列。Lea 基因包含真核基因的标准序列特征（TATA 盒和多聚（A）添加序列），并且具有 1 个或更多内含子。cDNA 和基因组 DNA 之间的序列差异证实了几个 Lea 基因的小多基因家族的存在。Lea 蛋白的氨基酸组成和序列很不寻常。其中 5 个蛋白具有极强的亲水性，4 个蛋白不含半胱氨酸或色氨酸，4 个蛋白具有暗示两亲性螺旋结构的序列结构域。基于氨基酸序列讨论了在干燥存活中的假设功能。
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