拟南芥4号染色体850 kb区域重复DNA的发生情况及性质分析。
Analysis of the occurrence and nature of repeated DNA in an 850 kb region of Arabidopsis thaliana chromosome 4.
作者信息
Thompson H L, Schmidt R, Dean C
机构信息
John Innes Centre, Norwich, UK.
出版信息
Plant Mol Biol. 1996 Nov;32(3):553-7. doi: 10.1007/BF00019109.
The occurrence and nature of repeated DNA sequences has been analysed within an 850 kb YAC contig on Arabidopsis thaliana chromosome 4. Hybridization analysis with seven RFLP markers, six cosmid contigs, 29 YAC end probes and eight YAC clones showed that a least 585 kb of the 850 kb contained only low-copy sequences. One YAC end probe, EG15C8LE, hybridized to multiple genomic fragments and contained a sequence with predicted protein homology to cytochrome P450 monooxygenases. Another one, EG11B7RE, was found to be non-contiguous with the other YAC clones and contained a dispersed repetitive sequence associated with centromeric regions.
对拟南芥4号染色体上一个850kb的酵母人工染色体（YAC）重叠群中的重复DNA序列的出现情况和性质进行了分析。用7个限制性片段长度多态性（RFLP）标记、6个黏粒重叠群、29个YAC末端探针和8个YAC克隆进行杂交分析，结果表明，在850kb中至少有585kb只包含低拷贝序列。一个YAC末端探针EG15C8LE与多个基因组片段杂交，并包含一个与细胞色素P450单加氧酶具有预测蛋白质同源性的序列。另一个探针EG11B7RE被发现与其他YAC克隆不连续，并包含一个与着丝粒区域相关的分散重复序列。
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