Bailey P C, Lycett G W, Roberts J A
Department of Physiology and Environmental Science, Faculty of Agricultural and Food Sciences, University of Nottingham, Leicestershire, UK.
Plant Mol Biol. 1996 Nov;32(3):559-64. doi: 10.1007/BF00019110.
A cDNA library was generated from seeds of Trollius ledebouri cv. Golden Queen after GA3 treatment. Five clones encoded mRNAs which were down-regulated during dormancy breaking and the initial stages of germination. Two of these showed homology to storage proteins (pPCB3 and pPCB4) and one each to the late-embryogenesis-abundant (LEA) group 2 dehydrin proteins (pPCB2), a barely glucose dehydrogenase (pPCB6) and the glutathione S-transferase (GST) superfamily (pPCB7). Transcript levels declined over 8 days in GA3-treated seeds. In dormant imbibed seeds transcript levels were relatively unchanged over the same period except for the PCB3 transcript, the level of which increased.
从经赤霉素（GA3）处理的金皇后毛茛种子中构建了一个cDNA文库。五个克隆编码的mRNA在休眠打破和萌发初期表达下调。其中两个与贮藏蛋白（pPCB3和pPCB4）具有同源性，另外三个分别与胚胎发育晚期丰富蛋白（LEA）第2组脱水蛋白（pPCB2）、一种大麦葡萄糖脱氢酶（pPCB6）和谷胱甘肽S-转移酶（GST）超家族（pPCB7）具有同源性。在GA3处理的种子中，转录水平在8天内下降。在休眠吸胀种子中，除了PCB3转录本水平升高外，同期转录水平相对不变。
