Nicolás C, Rodríguez D, Poulsen F, Eriksen E N, Nicolás G
Departamento de Biología Vegetal (Fisiología Vegetal), Facultad de Biología, Universidad de Salamanca, Spain.
Plant Cell Physiol. 1997 Dec;38(12):1303-10. doi: 10.1093/oxfordjournals.pcp.a029122.
By differential screening of a cDNA library constructed from poly (A+) RNA of ABA-treated seeds of Fagus sylvatica L., we have isolated an ABA-responsive clone that is present in dormant seeds and under conditions that maintain dormancy, but it tends to disappear under conditions breaking seed dormancy. A search of the sequence data bases showed that the clone codes for a Glycine-Rich Protein and has sequence similarity to RNA-binding proteins. The clone, which exibits the characteristics of lea-genes, is up-regulated by ABA and down-regulated by GA3. Paclobutrazol abolishes the effect of GA3, which is restored upon addition of GA3. The possible relationship of this Glycine-Rich Protein to seed dormancy in F. sylvatica is discussed.
通过对由欧洲水青冈经脱落酸（ABA）处理种子的聚腺苷酸（poly(A+)）RNA构建的cDNA文库进行差异筛选，我们分离出了一个ABA响应克隆，该克隆存在于休眠种子中以及维持休眠的条件下，但在打破种子休眠的条件下往往会消失。对序列数据库的搜索表明，该克隆编码一种富含甘氨酸的蛋白质，并且与RNA结合蛋白具有序列相似性。该克隆具有lea基因的特征，受ABA上调而受赤霉素（GA3）下调。多效唑消除了GA3的作用，添加GA3后作用得以恢复。本文讨论了这种富含甘氨酸的蛋白质与欧洲水青冈种子休眠之间的可能关系。
