Svensson E, Hanberger H, Nilsson L E
Department of Clinical Microbiology, Faculty of Health Sciences, University Hospital, Linköping, Sweden.
Antimicrob Agents Chemother. 1997 Jan;41(1):107-11. doi: 10.1128/AAC.41.1.107.
The pharmacodynamic effects of amikacin, imipenem, ofloxacin, rifampin, and vancomycin were studied on the slime-producing, oxacillin-resistant strain Staphylococcus epidermidis ATCC 35984 growing in Mueller Hinton broth or, in order to inhibit growth, incubated in phosphate-buffered saline. The investigated parameters were postantibiotic effect (PAE) and control-related effective regrowth time (CERT), which were determined by bioluminescence assay of bacterial ATP. PAE describes the delayed regrowth after drug removal, and CERT describes the combined effects of initial change in bacterial density during antibiotic exposure and delayed regrowth after drug removal. In growth cultures, PAE and CERT were drug concentration dependent for all antibiotics. The length of the PAE and CERT in the growing cultures were as follows: ofloxacin > rifampin > amikacin > vancomycin > imipenem. Imipenem combined with amikacin and vancomycin, respectively, induced a synergistic effect against growing cultures. In nongrowing cultures rifampin was the only drug that induced strong concentration-dependent effects. The combination of drugs induced no synergistic effects against nongrowing bacteria.
研究了丁胺卡那霉素、亚胺培南、氧氟沙星、利福平及万古霉素对在米勒-欣顿肉汤中生长或为抑制生长而在磷酸盐缓冲盐溶液中孵育的产黏液、耐苯唑西林的表皮葡萄球菌ATCC 35984的药效学作用。所研究的参数为抗生素后效应（PAE）和对照相关有效再生长时间（CERT），通过细菌ATP的生物发光测定来确定。PAE描述药物去除后生长延迟情况，CERT描述抗生素暴露期间细菌密度的初始变化及药物去除后生长延迟的综合效应。在生长培养物中，所有抗生素的PAE和CERT均呈药物浓度依赖性。生长培养物中PAE和CERT的时长如下：氧氟沙星＞利福平＞丁胺卡那霉素＞万古霉素＞亚胺培南。亚胺培南分别与丁胺卡那霉素和万古霉素联合，对生长培养物产生协同效应。在非生长培养物中，利福平是唯一诱导出强浓度依赖性效应的药物。药物联合对非生长细菌未产生协同效应。
