Pongsunk S, Ekpo P, Dharakul T
Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Asian Pac J Allergy Immunol. 1996 Jun;14(1):43-7.
Hybridomas secreting monoclonal antibodies (MAbs) specific to Burkholderia pseudomallei were produced by immunizing BALB/cJ mice with crude culture filtrate of B. pseudomallei. Two monoclonal antibodies were found to be highly specific to B.pseudomallei as tested by indirect enzyme-linked immunosorbent assay and immunoblotting against a panel of crude whole cell extracts from B. pseudomallei, B. cepacia, Pseudomonas aeruginosa, P.putida, and Escherichia coli. One of the specific MAbs, clone SP-M, IgM subclass, could directly agglutinate all 42 B. pseudomallei, isolates. The study has shown that the agglutinating MAb has potential for rapid identification of B. pseudomallei in primary bacterial culture from clinical specimens. The antibody can be used in bacteriology laboratories to reduce time of biochemical methods, which require a few days.
通过用伯克霍尔德菌粗培养滤液免疫BALB/cJ小鼠，制备出了分泌针对类鼻疽伯克霍尔德菌特异性单克隆抗体（MAb）的杂交瘤。通过间接酶联免疫吸附测定以及针对一组来自类鼻疽伯克霍尔德菌、洋葱伯克霍尔德菌、铜绿假单胞菌、恶臭假单胞菌和大肠杆菌的粗全细胞提取物进行免疫印迹检测，发现两种单克隆抗体对类鼻疽伯克霍尔德菌具有高度特异性。其中一种特异性单克隆抗体，克隆号为SP-M，IgM亚类，能够直接凝集所有42株类鼻疽伯克霍尔德菌分离株。该研究表明，这种具有凝集作用的单克隆抗体在从临床标本的原始细菌培养物中快速鉴定类鼻疽伯克霍尔德菌方面具有潜力。该抗体可用于细菌学实验室，以缩短需要几天时间的生化方法所需的时间。
