Carreira J C, Jones C, Wait R, Previato J O, Mendonça-Previato L
Instituto de Microbiologia, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Brasil.
Glycoconj J. 1996 Dec;13(6):955-66. doi: 10.1007/BF01053191.
The structures of the glycoinositolphospholipids (GIPLs) from five strains of the protozoan parasite Trypanosoma cruzi have been determined. Two series of structures were identified, all but one containing the same Man4(AEP)GlcN-Ins-PO4 core. Series 1 oligosaccharides are substituted at the third mannose distal to inositol (Man 3) by ethanolamine-phosphate or 2-aminoethylphosphonic acid, as are some glycosyl-phosphatidylinositol-protein anchors of T. cruzi. The core can be further substituted by terminal (1-3)-linked beta-galactofuranose units. In contrast, Series 2 oligosaccharides do not have additional phosphorus-containing groups attached to Man 3, the latter being substituted instead by a single side chain unit of beta-galactofuranose. Series 1 oligosaccharides are present in all strains (G, G-645, Tulahuen CL, and Y) whereas Series 2 structures are present mainly in CL and Y strains. The lipid moiety in the GIPLs from the G, G-645 and Tulahuen strains is predominantly ceramide, as reported for the Y strain, whilst that from the CL strain is a mixture of ceramide and alkylacylglycerol species. The lipid moiety of the GIPLs, and probably also the phosphoinositol-oligosaccharide structures may play an important immunomodulatory role in infection by T. cruzi.
已确定来自原生动物寄生虫克氏锥虫五个菌株的糖基肌醇磷脂（GIPL）的结构。鉴定出了两个系列的结构，除一种外，所有结构都含有相同的Man4(AEP)GlcN-Ins-PO4核心。系列1寡糖在距肌醇第三远的甘露糖（Man 3）处被磷酸乙醇胺或2-氨基乙基膦酸取代，克氏锥虫的一些糖基磷脂酰肌醇-蛋白质锚也是如此。核心可被末端（1-3）连接的β-呋喃半乳糖单元进一步取代。相比之下，系列2寡糖在Man 3上没有额外的含磷基团连接，后者被单个β-呋喃半乳糖侧链单元取代。系列1寡糖存在于所有菌株（G、G-645、图拉温CL和Y）中，而系列2结构主要存在于CL和Y菌株中。G、G-645和图拉温菌株的GIPL中的脂质部分主要是神经酰胺，如Y菌株报道的那样，而CL菌株的脂质部分是神经酰胺和烷基酰基甘油种类的混合物。GIPL的脂质部分，可能还有磷酸肌醇寡糖结构，在克氏锥虫感染中可能起重要的免疫调节作用。
