Banic S
Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Slovenia.
J Chemother. 1996 Dec;8(6):432-7. doi: 10.1179/joc.1996.8.6.432.
We have found that azide-resistant mutants of Salmonella typhimurium and of other bacteria studied produce a substance which inactivates the azide. The production of this substance was proved by the demonstration of a satellite growth of azide-sensitive cells around colonies of azide-resistant mutants and by testing azide inactivating properties of culture filtrates of the azide-resistant strains. The same substance was found to be present in lower concentrations in culture filtrates of wild-type sensitive strains. In both cultures of sensitive strains, it was apparently produced by the resistant mutants and not by the sensitive cells. The substance does not pass across a dialysis membrane and is heat stable. It has a high molecular weight but is not a protein.
我们发现，鼠伤寒沙门氏菌及其他所研究细菌的叠氮化物抗性突变体产生一种能使叠氮化物失活的物质。通过证明叠氮化物敏感细胞在叠氮化物抗性突变体菌落周围的卫星生长，以及检测叠氮化物抗性菌株培养滤液的叠氮化物失活特性，证实了这种物质的产生。在野生型敏感菌株的培养滤液中也发现了浓度较低的相同物质。在敏感菌株的两种培养物中，这种物质显然是由抗性突变体产生的，而非敏感细胞。该物质不能透过透析膜，且耐热。它具有高分子量，但不是蛋白质。
