Cieśla Z, Mardarowicz K, Klopotowski T
Mol Gen Genet. 1974;135(4):339-48. doi: 10.1007/BF00271148.
Evidence has been obtained that sodium azide is an inhibitor of cell division in wild-type and aziA strains of Salmonella typhimurium. The bacteria grown in media containing sodium azide and glucose formed long filaments. It has been found that sodium azide had a stronger inhibitory effect on DNA synthesis than on cell mass increase. When filaments produced by azide action were transferred to azide-free medium very rapid increase in DNA content was observed during the first 45 min. After this time, when relative DNA content was increased the rate of DNA synthesis was reduced and cell divisions reappeared. Inhibitory effect of azide on DNA biosynthesis in vitro was observed with toluenized cells of S typhimurium. Only ATP-dependent radioactive dTMP incorporation into DNA was affected by sodium azide. It had no effect on the incorporation in the absence of ATP. Mutant aziC was isolated in S. typhimurium by scoring for clones with normal cell division in the presence of sodium azide. Azide had much less effect on DNA biosynthesis in vivo and in vitro in aziC cells as compared with isogenic controls.
已获得证据表明，叠氮化钠是鼠伤寒沙门氏菌野生型和aziA菌株中细胞分裂的抑制剂。在含有叠氮化钠和葡萄糖的培养基中生长的细菌形成了长丝。已发现叠氮化钠对DNA合成的抑制作用比对细胞质量增加的抑制作用更强。当由叠氮化物作用产生的丝状体转移到无叠氮化物的培养基中时，在最初的45分钟内观察到DNA含量迅速增加。在此之后，当相对DNA含量增加时，DNA合成速率降低，细胞分裂重新出现。用鼠伤寒沙门氏菌的甲苯化细胞观察到叠氮化物对体外DNA生物合成的抑制作用。只有依赖ATP的放射性dTMP掺入DNA受到叠氮化钠的影响。在没有ATP的情况下，它对掺入没有影响。通过对在叠氮化钠存在下具有正常细胞分裂的克隆进行评分，在鼠伤寒沙门氏菌中分离出突变体aziC。与同基因对照相比，叠氮化物对aziC细胞体内和体外DNA生物合成的影响要小得多。
