使用多重引物通过聚合酶链反应（PCR）检测和鉴定鼠疫耶尔森菌。
Detection and identification of Yersinia pestis by polymerase chain reaction (PCR) using multiplex primers.
作者信息
Tsukano H, Itoh K, Suzuki S, Watanabe H
机构信息
Department of Bacteriology, National Institute of Health, Tokyo, Japan.
出版信息
Microbiol Immunol. 1996;40(10):773-5. doi: 10.1111/j.1348-0421.1996.tb01140.x.
A PCR method for detection of Yersinia pestis-virulence determinants by the use of multiplex primers was developed. Four pairs of oligonucleotide primers were designed from each gene of three kinds of virulent plasmids and a chromosomal DNA; 60-Md plasmid-located gene (caf1)encoding Y.pestis-specific capsular antigen fraction 1, a Y.pestis-specific region of a yopM gene encoded on 42-Md virulent plasmid, a plasminogen activator gene (pla) encoded on Y.pestis-specific 7-Md plasmid and an invasin protein gene (inv) encoded on chromosomal DNA. This multiplex-primer system was specific for the detection of Y.pestis among pathogenic Yersinia species and other enterobacteriaceae having antigens common to Y.pestis. Since this method is simple and safe, it will be useful to identify and confirm Y.pestis in cases of emergency and for the surveillance of epidemics.
开发了一种利用多重引物检测鼠疫耶尔森菌毒力决定因素的聚合酶链反应（PCR）方法。从三种毒性质粒和一种染色体DNA的每个基因设计了四对寡核苷酸引物；位于60-Md质粒上的编码鼠疫耶尔森菌特异性荚膜抗原组分1的基因（caf1）、位于42-Md毒性质粒上的yopM基因的鼠疫耶尔森菌特异性区域、位于鼠疫耶尔森菌特异性7-Md质粒上的纤溶酶原激活剂基因（pla）以及位于染色体DNA上的侵袭蛋白基因（inv）。这种多重引物系统对于在致病性耶尔森菌属物种和具有与鼠疫耶尔森菌共同抗原的其他肠杆菌科细菌中检测鼠疫耶尔森菌具有特异性。由于该方法简单且安全，在紧急情况下识别和确认鼠疫耶尔森菌以及进行疫情监测方面将很有用。
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