通过巢式聚合酶链反应程序快速、特异性鉴定鼠疫耶尔森菌。
Rapid and specific identification of Yersinia pestis by using a nested polymerase chain reaction procedure.
作者信息
Campbell J, Lowe J, Walz S, Ezzell J
机构信息
Naval Research Laboratory, Washington, D.C. 20375.
出版信息
J Clin Microbiol. 1993 Mar;31(3):758-9. doi: 10.1128/jcm.31.3.758-759.1993.
Abstract
We developed a 4-h nested polymerase chain reaction assay that detected a region of the plasminogen activator gene of Yersinia pestis in 100% of 43 Y. pestis strains isolated from humans, rats, and fleas yet was unreactive with the closely related species Yersinia enterocolitica and Yersinia pseudotuberculosis.
摘要
我们开发了一种4小时嵌套聚合酶链反应检测方法，该方法在从人类、大鼠和跳蚤中分离出的43株鼠疫耶尔森菌中，100%检测到了鼠疫耶尔森菌纤溶酶原激活剂基因的一个区域，但与密切相关的小肠结肠炎耶尔森菌和假结核耶尔森菌无反应。
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