霍乱弧菌磷酸赤藓糖脱氢酶基因的鉴定、测序及酶活性分析
Identification, sequencing, and enzymatic activity of the erythrose-4-phosphate dehydrogenase gene of Vibrio cholerae.
作者信息
Carroll P A, Zhao G, Boyko S A, Winkler M E, Calderwood S B
机构信息
Infectious Disease Unit, Massachusetts General Hospital, Boston 02114, USA.
出版信息
J Bacteriol. 1997 Jan;179(1):293-6. doi: 10.1128/jb.179.1.293-296.1997.
Abstract
We have identified a gene in Vibrio cholerae (epd) which encodes an erythrose-4-phosphate dehydrogenase activity and is located immediately downstream of an iron-regulated virulence gene, irgA, and immediately upstream of a gene encoding phosphoglycerate kinase (pgk). Expression of epd in V. cholerae is not regulated by iron, nor is it required for virulence in an infant mouse model.
摘要
我们在霍乱弧菌中鉴定出一个基因（epd），它编码4-磷酸赤藓糖脱氢酶活性，位于铁调节毒力基因irgA的紧下游，以及编码磷酸甘油酸激酶（pgk）的基因的紧上游。霍乱弧菌中epd的表达不受铁的调节，在幼鼠模型中其毒力也不需要该基因。
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