Alefounder P R, Perham R N
Department of Biochemistry, University of Cambridge, UK.
Mol Microbiol. 1989 Jun;3(6):723-32. doi: 10.1111/j.1365-2958.1989.tb00221.x.
To investigate a possible chromosomal clustering of glycolytic enzyme genes, the complete nucleotide sequence of the 8029 bp insert of Escherichia coli DNA in the ColE1 plasmid pLC33-5 of the Clarke and Carbon collection (Clark and Carbon, 1976) was determined. Genes (pgk, fda) encoding the phosphoglycerate kinase and Class II fructose 1,6-bisphosphate aldolase, respectively, of E. coli were identified. The phosphoglycerate kinase was found to be highly homologous in primary structure to the same enzyme from eukaryotic organisms. A further large open reading frame, designated gapB, was also identified, which on the basis of sequence homology, appears to encode another glycolytic enzyme, glyceraldehyde 3-phosphate dehydrogenase. This putative gene differs significantly from that (designated gapA) already identified as coding for this enzyme in E. coli and which maps elsewhere on the chromosome. The products, if any, of several other open reading frames remain to be identified.
为了研究糖酵解酶基因可能的染色体聚类情况，测定了克拉克和卡尔文文库（Clark和Carbon，1976）中大肠杆菌DNA插入ColE1质粒pLC33 - 5的8029 bp片段的完整核苷酸序列。鉴定出了分别编码大肠杆菌磷酸甘油酸激酶和II类果糖1,6 - 二磷酸醛缩酶的基因（pgk，fda）。发现磷酸甘油酸激酶在一级结构上与真核生物中的同一种酶高度同源。还鉴定出了另一个大的开放阅读框，命名为gapB，根据序列同源性，它似乎编码另一种糖酵解酶，即甘油醛 - 3 - 磷酸脱氢酶。这个推测的基因与已确定在大肠杆菌中编码该酶且位于染色体其他位置的基因（命名为gapA）有显著差异。其他几个开放阅读框的产物（如果有的话）仍有待鉴定。
