Bertrand E, Devreker F, van den Bergh M, Englert Y
Dept. Obstetrics and Gynecology, Erasme Hospital, Free University of Brussels, Belgium.
Eur J Morphol. 1996 Nov;34(4):245-55. doi: 10.1076/ejom.34.4.245.13042.
The present study evaluates the usefulness of the sub-zonal insemination (SUZI) for patients with previous IVF failures or affected by severe oligoasthenoteratozoospermia and the necessity of pre-treatment semen analysis.
Twenty-five patients underwent 43 cycles of either SUZI or alternate SUZI/IVF procedures according to semen characteristics.
Among the 571 retrieved oocytes, 398 were microinjected and 173 were treated by IVF. Among the microinjected oocytes, 19% fertilized (12% 2PN: 7% 3PN) and 96% of the 2PN oocytes developed into embryos. Thirty-one embryo transfers were performed, 8 pregnancies were obtained and 7 embryos were cryopreserved. The 2 PN rates in SUZI and IVF procedures in the same cycle were not significantly different. The analysis of the different semen samples obtained before and during the treatment reveals that the semen quality varies in such a way that the decision of the adequate microinjection strategy to follow should only be taken according to the semen quality at the day of treatment.
SUZI is not a satisfactory technique for treatment neither in case of previous IVF failures nor for severe oligoasthenoteratozoospermia.
本研究评估分区内授精（SUZI）对既往体外受精（IVF）失败患者或重度少弱畸精子症患者的有效性以及治疗前精液分析的必要性。
25例患者根据精液特征接受了43个周期的SUZI或交替的SUZI/IVF程序。
在回收的571个卵母细胞中，398个进行了显微注射，173个接受了IVF治疗。在显微注射的卵母细胞中，19%受精（12%为2PN：7%为3PN），96%的2PN卵母细胞发育成胚胎。进行了31次胚胎移植，获得8次妊娠，7个胚胎被冷冻保存。同一周期中SUZI和IVF程序的2PN率无显著差异。对治疗前和治疗期间获得的不同精液样本的分析表明，精液质量存在差异，因此应仅根据治疗当天的精液质量来决定采用何种合适的显微注射策略。
对于既往IVF失败的情况或重度少弱畸精子症，SUZI都不是一种令人满意的治疗技术。
