Damgé C, Aprahamian M, Marchais H, Benoit J P, Pinget M
CEED Hôpitaux Universitaires, Strasbourg, France.
J Anat. 1996 Dec;189 ( Pt 3)(Pt 3):491-501.
Rhodamine B-labelled poly (DL-lactide-co-glycolide) (PLAGA) microspheres of 2 different sizes, 1-5 microns and 5-10 microns, were administered as a single dose (1.44 x 10(9) and 1.83 x 10(8) particles, respectively) into the ileal lumen of adult rats. The content of rhodamine in the mesenteric vein and ileal lumen was analysed periodically from 10 min to 48 h as well as the distribution of microspheres in the intestinal mucosa and various other tissues. The concentration of rhodamine decreased progressively in the intestinal lumen and was negligible after 24 h. The number of microspheres in the mesenteric vein increased rapidly and reached a maximum after 4 h whatever the size of the particles. It then decreased progressively, but more rapidly with microspheres > 5 microns than with microspheres < 5 microns. The absorption efficiency was low for the former batch (about 0.11% of the administered dose) and higher for the latter (about 12.7%). The intraileal administration of free rhodamine B was followed by intense labelling of the epithelial cells and basement membranes in mesenteric lymph nodes, spleen, kidney and liver. PLAGA microspheres mainly crossed the intestinal mucosa at the site of Peyer's patches where microspheres of < 5 microns appeared after 3 h. Microspheres > 5 microns were retained in the ileal lumen. A few small microspheres were occasionally observed in the epithelial cells. Only the smallest particles were recovered in the liver, lymph nodes and spleen while basement membranes were always labelled. It is concluded that PLAGA microspheres could be useful for the oral delivery of antigens if their size is between 1 and 5 microns.
将两种不同大小（1 - 5微米和5 - 10微米）的罗丹明B标记的聚（DL - 丙交酯 - 乙交酯）（PLAGA）微球作为单剂量（分别为1.44×10⁹和1.83×10⁸个颗粒）给予成年大鼠的回肠腔。从10分钟到48小时定期分析肠系膜静脉和回肠腔中罗丹明的含量，以及微球在肠黏膜和其他各种组织中的分布。肠腔中罗丹明的浓度逐渐降低，24小时后可忽略不计。无论颗粒大小如何，肠系膜静脉中的微球数量迅速增加，并在4小时后达到最大值。然后逐渐减少，但大于5微米的微球比小于5微米的微球减少得更快。前一批微球的吸收效率较低（约为给药剂量的0.11%），而后一批微球的吸收效率较高（约为12.7%）。回肠内给予游离罗丹明B后，肠系膜淋巴结、脾脏、肾脏和肝脏中的上皮细胞和基底膜出现强烈标记。PLAGA微球主要在派尔集合淋巴结部位穿过肠黏膜，3小时后出现小于5微米的微球。大于5微米的微球保留在回肠腔中。偶尔在上皮细胞中观察到一些小的微球。只有最小的颗粒在肝脏、淋巴结和脾脏中被回收，而基底膜总是被标记。结论是，如果PLAGA微球的大小在1至5微米之间，可用于口服递送抗原。
