Hazzard R A, Hodges G M, Scott J D, McGuinness C B, Carr K E
School of Biomedical Science/Anatomy, Queen's University of Belfast, Northern Ireland, UK.
J Anat. 1996 Oct;189 ( Pt 2)(Pt 2):265-71.
This time-course study investigates the early uptake and passage of microparticles across the intestinal mucosa. Single intraoral doses of fluorescent latex particles, 1.82 microns in diameter, were administered to young adult male, nonfasted rats at a dose of 1.88 x 10(9) particles. Peyer's patch regions and mesenteric lymph nodes were collected at 5, 15 and 30 min time points for both bulk tissue and morphological analyses. Particles were found at all experimental time points in macerated intestinal and nodal specimens: particle numbers were higher in proximal than in distal intestine at all time points despite the fact that particle numbers in distal Peyer's patch regions increased with time. Particle numbers in mesenteric lymph nodes also increased with time after administration. Detailed morphological data for several intestinal and nodal tissue compartments showed substantial early uptake of particles by villous epithelium, including goblet cells, but low involvement of follicle-associated cells. The distribution of particles in the lymph nodes confirmed that translocation occurred to all nodal compartments. These studies give confirmatory evidence that uptake and translocation of microparticles may take place as early as 5 min after administration and suggest that intestinal region may influence uptake.
这项时间进程研究调查了微粒在肠道黏膜的早期摄取和通过情况。给未禁食的成年雄性大鼠经口单次给予直径为1.82微米的荧光乳胶微粒，剂量为1.88×10⁹个微粒。在5分钟、15分钟和30分钟时间点收集派尔集合淋巴结区域和肠系膜淋巴结，用于整体组织和形态学分析。在所有实验时间点，在研磨后的肠道和淋巴结标本中均发现了微粒：尽管远端派尔集合淋巴结区域的微粒数量随时间增加，但在所有时间点近端肠道中的微粒数量均高于远端肠道。给药后肠系膜淋巴结中的微粒数量也随时间增加。几个肠道和淋巴结组织区室的详细形态学数据显示，绒毛上皮，包括杯状细胞，对微粒有大量早期摄取，但滤泡相关细胞的参与度较低。微粒在淋巴结中的分布证实其转运至所有淋巴结区室。这些研究提供了确证性证据，表明微粒的摄取和转运可能在给药后5分钟就开始发生，并提示肠道区域可能影响摄取。
