Zashikhin A L, Lisishnikov L V, Agafonov Iu V
Morfologiia. 1996;109(3):64-6.
The present research was aimed to study the dynamics of changes of bronchial smooth myocytes morphometric and cytospectrophotometric indexes in thanatogenesis. Selected dissociation of albino outbred rat bronchial smooth myocytes was performed right after animals were killed (3, 6, 12, 24 and 48 hrs). Smears of the dissociated myocytes, stained after Feulgen to be revealed on DNA and of amid black stained general protein were examined by scanning cytospectrophotometer. Morphometric parameters of nucleus and cytoplasm were determined simultaneously. Continuous decrease of optical density of summary protein and DNA, corresponding with data from literature was found. Volume of myocyte cytoplasm significantly grows smaller within first 12 postmortem hrs, which results from population structure alteration due to elimination of cells with basically large cytoplasmic volume. Thus not only dynamics of cytochemical and linear parameters changes in cells should be taken into consideration in postmortem histological analysis of the lung material but also the changes of cell population structure.
本研究旨在探讨支气管平滑肌细胞形态计量学和细胞分光光度学指标在死亡过程中的动态变化。选用白化远交系大鼠，在动物处死后立即（3、6、12、24和48小时）进行支气管平滑肌细胞的解离。解离后的肌细胞涂片，经福尔根染色以显示DNA，并经酰胺黑染色以显示总蛋白，然后用扫描细胞分光光度计进行检测。同时测定细胞核和细胞质的形态计量参数。发现总蛋白和DNA的光密度持续下降，这与文献数据相符。在死后的前12小时内，肌细胞质体积显著减小，这是由于大量细胞质体积的细胞被清除导致细胞群体结构改变所致。因此，在肺组织材料的死后组织学分析中，不仅应考虑细胞内细胞化学和线性参数变化的动态，还应考虑细胞群体结构的变化。
