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偶蹄目动物中随机扩增多态性DNA片段的DNA序列

DNA sequences of RAPD fragments in artiodactyls.

作者信息

Kostia S, Palo J, Varvio S L

机构信息

Department of Biosciences, University of Helsinki, Finland.

出版信息

Genome. 1996 Apr;39(2):456-8. doi: 10.1139/g96-057.

Abstract

A bovine RAPD profile, generated by a 10-mer primer, was analysed by sequencing the major fragments. Three of four different fragments showed homologies to previously characterized mammalian sequences. One was 61-66% identical to LINE sequences and another was 78.5% identical to a human chromosome 2 sequence tagged site. The third fragment was 93.1% identical to the human type 2 inositol 1,4,5-trisphosphate receptor gene. This fragment had counterparts in white-tailed deer and reindeer; fragments of slightly different size in these species showed high sequence similarity and the size differences were due to varying numbers of dinucleotide microsatellite repeats inside the fragment.

摘要

通过一个10聚体引物生成的牛随机扩增多态性DNA图谱,对主要片段进行测序分析。四个不同片段中的三个与先前鉴定的哺乳动物序列具有同源性。一个与长散布核元件序列有61%-66%的同一性,另一个与人类2号染色体序列标签位点有78.5%的同一性。第三个片段与人类2型肌醇1,4,5-三磷酸受体基因有93.1%的同一性。该片段在白尾鹿和驯鹿中有对应物;这些物种中大小略有不同的片段显示出高度的序列相似性,大小差异是由于片段内二核苷酸微卫星重复序列数量的不同。

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