Lima-de-Faria A, Arnason U, Widegren B, Essen-Möller J, Isaksson M, Olsson E, Jaworska H
J Mol Evol. 1984;20(1):17-24. doi: 10.1007/BF02101981.
The Cervidae show one of the largest variations in chromosome number found within a mammalian family. The five species of the deer family which are the subject of this study vary in chromosome number from 2n = 70 to 2n = 6. Digestion with the restriction enzymes EcoRI, HpaII, HaeIII and MspI reveals that there is a series of highly repetitive sequences forming similar band patterns in the different species. To obtain information on the degree of homology among these conserved sequences we isolated a HpaII restriction fragment of approximately 990 base pairs from reindeer DNA. This DNA sequence was 32P-labelled and hybridized by the Southern blot technique to DNAs cleaved with HpaII and HaeIII from the reindeer and four other Cervidae species. Hybridization to specific restriction fragments was recorded in all species. The patterns of hybridization showed a higher degree of similarity between reindeer, elk and roe deer than between reindeer and the Asiatic species (fallow deer and muntjac). Homologies are still present between the highly repetitive sequences of the five species despite the drastic reorganization that led to a change in chromosome number from 6 to 70.
鹿科动物的染色体数目变化是哺乳动物家族中最大的之一。本研究的对象是鹿科的五个物种，其染色体数目从2n = 70到2n = 6不等。用限制性内切酶EcoRI、HpaII、HaeIII和MspI进行消化后发现，不同物种中存在一系列形成相似条带模式的高度重复序列。为了获得这些保守序列之间的同源程度信息，我们从驯鹿DNA中分离出一个约990个碱基对的HpaII限制性片段。该DNA序列用32P标记，并通过Southern印迹技术与驯鹿以及其他四种鹿科动物经HpaII和HaeIII切割的DNA杂交。在所有物种中均记录到与特定限制性片段的杂交情况。杂交模式显示，驯鹿、驼鹿和狍之间的相似程度高于驯鹿与亚洲物种（黇鹿和麂）之间的相似程度。尽管发生了导致染色体数目从6变为70的剧烈重组，但这五个物种的高度重复序列之间仍然存在同源性。
