Nishikawa S, Sasaki F
Department of Biology, Tsurumi University, School of Dental Medicine, Yokohama, Japan.
J Histochem Cytochem. 1996 Dec;44(12):1459-67. doi: 10.1177/44.12.8985138.
We examined the processing of apoptotic ameloblast fragments at the transitional stage by immunocytochemistry and electron microscopy. Both macrophages and epithelial cells in the enamel organ ingested apoptotic bodies. MHC Class II antigen expression and the phagolysosomal nature of the macrophages were further examined by immunoelectron microscopy using OX6 and ED1 antibodies followed by HRP- or gold-conjugated secondary antibodies. The results showed that macrophages in the enamel organ at the transitional stage expressed MHC Class II antigens at their plasma membranes and that the same cells contained apoptotic fragments in ED1-positive vacuoles. We suggest that diverse immunological responses, such as the self-antigen presentation, may be invoked in the process of apoptosis.
我们通过免疫细胞化学和电子显微镜检查了过渡阶段凋亡成釉细胞碎片的处理情况。牙釉质器官中的巨噬细胞和上皮细胞均摄取了凋亡小体。使用OX6和ED1抗体，继以辣根过氧化物酶或金标记的二抗，通过免疫电子显微镜进一步检查巨噬细胞的MHC II类抗原表达和吞噬溶酶体性质。结果显示，过渡阶段牙釉质器官中的巨噬细胞在其质膜上表达MHC II类抗原，并且相同的细胞在ED1阳性液泡中含有凋亡碎片。我们认为，在凋亡过程中可能引发多种免疫反应，例如自身抗原呈递。
